Don't expect "Plug and Play." You’ll manually need to configure Sound Blaster 16 drivers in the SYSTEM.INI file if they aren't pre-baked into your ISO.
To make your ISO boot directly into Windows 3.1, edit C:\AUTOEXEC.BAT and add: windows 3.1 bootable iso download
As of 2026, the Windows 3.1 community remains active. Projects like (for Linux) and vDos have added better support for 16-bit Windows applications. Meanwhile, Microsoft’s open-sourcing of older software (like MS-DOS 1.25/2.0) raises hope that one day Windows 3.1 might be officially released as freeware. Don't expect "Plug and Play